Allamanda cathartica L. latex mediated magnesium oxide nanoparticles as antiproliferative agents

Abstract

Green based nanoparticles were synthesized to investigate the cytotoxic effects on human peripheral blood mononuclear cells (hPBMCs). Nanoparticles were made by mixing aqueous Allamanda cathartica L. latex (3%) and magnesium oxide (1mM) at 37oC on shaker for 24 hours. The developed nanoparticles were characterized by Scanning Electron Microscopy, Transmission Electron Microscopy, Fourier Transmission Infra-Red, Zeta potential and X-ray diffraction. hPBMCs were tested in vitro with different concentrations of synthesized nanoparticles and found that 100 μg/mL showed about 96% dead cells from trypan blue dye exclusion method. Though controlling the proliferation of cells, the nanoparticles did not exhibit any genotoxicity, indicating that the nanoparticles showed their effect on the periphery of the cells, breaking them and leading to cell death. This is the first report of green route development of MgO nanoparticles using the latex of Allamanda cathartica L. and finding their efficacy on hPBMCs. Further analysis is required to confirm their use as therapeutics in leukemia.