Evaluation of lipid peroxidation, protein carbonyl content and total antioxidant status in pre and post – delivery of women with preeclampsia

Abstract

Preeclampsia is a metabolic syndrome and a major cause of maternal, fetal and neonatal morbidity and mortality that amounts to 10% of the pregnancy complications. To determine Malondialdehyde, protein carbonyl and total antioxidant status in preeclampsia and normal pregnant women during pre and post labour within 48 hours. A prospective case control study (n= 60), preeclampsia (n=30) and normal pregnant women (n=30) during pre and post labour within 48 hours. 3 ml of blood samples were collected during pre and post labour of normal pregnant and preeclampsia. MDA, Protein Carbonyl Content and Total Antioxidant Status were measured using spectrophotometric method. Mean ± SD were determined by using student “t” test. The Mean ± SD values of MDA µmol/L (4.7 ±1.8), Protein Carbonyls (168.9±70.5) nmol/, Total Antioxidant Status mmol/L (537 ± 451) in normal pregnant during pre-delivery and MDA µmol/L(2.4 ±0.1), Protein Carbonyls (169±67.2)nmol/L , Total Antioxidant Status mmol/L (634.3 ± 241.2) post-delivery presented. Similarly Mean ± SD values of MDA µmol/L (10.1 ±6.4), Protein Carbonyls (159±123.2)nmol/L , Total Antioxidant Status mmol/L (506.7 ± 287.6) in preeclampsia pre delivery and (6.1±6.1), (98.8±36.8), (680±362.3) post-delivery respectively. A well-known aspect in preeclampsia with increased concentrations of oxidative stress and decreased TAS. However the trend of increased TAS and declined oxidative stress during post labour of normal pregnant and preeclampsia which plays a significant role in pathophysiology of preeclampsia.